小针电凝对SD大鼠真皮成纤维细胞活性影响的初步研究(1)
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[摘要]目的：研究小针电凝对SD大鼠真皮成纤维细胞生长活性的影响，并探讨其可能的发生机制。方法：应用小针高频电凝分别在6J/H、8J/H、10J/H、12J/H功率下对SD大鼠真皮进行选择性电热解作用2s，然后分别在术后第14天、第35天取标本制成病理切片光镜下观察成纤维细胞和胶原纤维的数量变化。结果：经小针高频电凝作用后第35天的SD大鼠真皮中胶原纤维数量明显增加(P<0.05)。结论：小针高频电凝的适度选择性电热解作用可增加SD大鼠真皮中成纤维细胞的活性，增加其对胶原纤维的分泌，从而提示小针高频电凝对人体皮肤有非剥脱性除皱的作用。
[关键词]小针电凝；成纤维细胞；胶原纤维；非剥脱性除皱
[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2011)05-0759-02
Initial research for the cytoactive of the fibroblast in the dermis of SD mice after treatment by fine-needle electrocoagulation
YU Hai-sheng,CHEN Shi-hai,LIU Qing-feng,WEI Qiang,LIAO Ming-de,MENG Xu-chang
(Department of Plastic Surgery,The First Affiliated Hospital of Guangxi Medical University, Nanning 530021,Guangxi,China)
Abstract:ObjectiveTo research the effects of the cytoactive of the Fibroblast in the dermis of SD Mice after selectional treatment by Fine-needle electrocoagulation,and infer the mechanism that results in the effects.MethodsUsing Fine-needle，the Dermis of SD Mice were electrolyzed selectionally at 6J/H、8J/H、10J/H、and 12J/H power.Then the specime,electrolyzed with Fine-needle,were made into the pathological sections on 14 and 35 days after electrocoagulation，the change of the fibroglia fibrils and the fibroblast would be observeed in photon microscope.ResultsThe quantity of the fibroglia fibrils in the the dermis of SD mice were more incremental on 35 days after electrocoagulation than before that(P<0.05).ConclusionsThe quantity of the fibroglia fibrils that secreted by the Fibroblast，were increase in the dermis of the SD mice after moderately selectional electrocoagulation，and what manifest that the effects of nonablative skin rejuvenation can be obtained by electrocoagulation.
Key words:Fine-needle electrocoagulation;fibroblast;fibroglia fibrils;nonablative skin rejuvenation
笔者在临床应用小针电凝治疗皮肤血管瘤时发现，该方法治疗后的部分皮肤不仅无瘢痕形成，而且皮肤皱纹较周围明显减少。本实验研究了小针高频电凝对SD大鼠真皮成纤维细胞活性和胶原纤维数量的影响，为小针高频电凝对皮肤非剥脱性除皱提供理论依据。
1资料和方法
1.1 试剂和所用仪器：华佗牌针灸针，上海沪通电子有限公司GD350-B型高频电刀作为治疗电凝器，光学显微镜等。
1.2 试验方法：选8只体重(120±10)g的雄性SD大鼠，随机分成4组(A～D组)，选右侧背部1.5cm×1.5cm面积为实验侧，左侧对应部位为对照侧，在25%乌拉坦麻醉下将针灸针平行皮肤刺入大鼠实验区的真皮内约1.5cm，针间距为0.3cm，A～D组分别在6J/h、8J/h、10J/h、12J/h功率下应用电凝器电凝2s，术后电凝区及对照区皮肤均涂以金霉素眼膏。分别在术后14天，35天取实验区和对照区皮肤标本，采用masson染色法制成病理切片光镜下观察、对比，采用Image-ProPlus图像分析软件进行半定量分析，每张切片取IOD作为其含量的测量值。
胶原纤维的半定量分析采用t检验，结果以平均数士标准差(x±s)表示。检验水准取a=0.05，P<0.05为有统计学意义 ......
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