骨髓间充质干细胞对自然衰老小鼠T细胞的调节作用研究(1)
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[摘要]目的：研究探讨小鼠骨髓间充质干细胞对自然衰老小鼠T细胞的节作用影响情况。方法：通过流式细胞术研究对比衰老小鼠和年轻小鼠T细胞培养48h后CD8+CD28+的共表达率差异，根据以上研究结果分别对比隔离共培养组、混合共培养组和对照组之间T细胞上CD8+CD28+的共表达率差异。结果：实验一中衰老组培养48h后CD8+CD28+共表达率为(4.08±0.3493)%，年轻组培养48h后CD8+CD28+共表达率为(5.04±0.5741)%。两者相比有统计学差异(P<0.05)。实验二隔离共培养组中骨髓间充质干细胞与淋巴细胞以1:1在Transwell系统中共培养，48h后其对应的CD8+CD28+共表达率为(10.26±1.0784)%，混合共培养组中骨髓间充质干细胞与淋巴细胞以1:1比例在六孔板中共培养，48h后CD8+CD28+共表达率为(6.42±1.5680)%。对照组中淋巴细胞以106数量进行培养，48h后CD8+CD28+共表达率为(4.08±0.3493)%。两两对比有统计学差异(P<0.05)。结论：衰老小鼠和年轻小鼠的T细胞在CD8+CD28+的共表达比例上存在差异。骨髓间充质干细胞能够通过分泌的某些细胞因子使这种衰老的T细胞变得趋向于“年轻态”。
[关键词]骨髓间充质干细胞；T淋巴细胞；免疫衰老；流式细胞术
[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2011)05-0761-04
Study on effect of bone marrou mesenchymal stem cells on aging mouse T cells regulation
ZHANG Lei1,SHI Bing-bing1,ZHOU Bi-yu2,YAN Li1,LI Yan1,CAO Yi-mei2,ZHAO Zhen-min2
(1.Research Center,2.The First Department,Plastic Surgery Hospital,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100144,China)
Abstract:ObjectiveThis is a study on effect of mouse BM-MSCs on aging mouse T cell regulation. Methods There were two groups in experiment one, aging group and young group ,the difference of CD8+ CD28+ expression between the two groups were detected with flow cytometry,according to the result ofexperiment one, the difference of CD8 CD28 expression of separated co-culture group, mixed co-culture group and control group,which were called experiment two,was detected with flow cytometry. ResultsIn the aging group of experiment one,the CD8+ CD28+ expression was (4.08±0.3493)%.the CD8+ CD28+ expression of young group was (5.04±0.5741)%.There was a statistical difference.In the separated co-culture group of experiment two,we co-cultured BM-MSCs and lymphocyte in accordance with the proportion of 1:1，and the CD8+ CD28+ expression was (10.26±1.0784)% after 48 hours. In the mixed co-culture,we mixed BM-MSCs with lymphocyte in 6 wells plate in accordance with the proportion of 1:1,and the CD8+ CD28+ expression was (6.42±1.5680)% after 48 hours ......
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