乙肝病毒携带产妇乳汁乙肝病毒标志物检测及分析(1)
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【摘要】 目的 通过检测乙肝血清学指标阳性的产妇乳汁中乙肝两对半及外周血HBV-DNA定量，以探讨两者之间的关系及其对于母乳喂养的影响。方法 对42例血清乙肝指标阳性的产妇，应用酶联免疫吸附法和荧光定量法检测乳汁中乙肝两对半及血HBV-DNA，42例血清乙肝指标阴性产妇作为对照组。结果 42例HBV携带孕妇产后乳汁乙肝两对半HbsAg检出率为33.3%。42例HBV携带孕妇HBV-DNA阳性率为28.57%。对照组乳汁HbsAg检出率为0，HBV-DNA阳性率为0。结论 乙肝小三阳或仅HbsAg阳性的孕妇乳汁乙肝病毒检出率低，母乳喂养相对安全，并且其乳汁的传染性明显低于乙肝大三阳孕妇。乳汁乙肝两对半全阴且血清HBV-DNA处于正常范围的产妇提倡母乳喂养。
【关键词】乙型肝炎病毒；人；母乳喂养
Research on hepatitis B virus markers in breast milk of hepatitis B virus carried mothers
WANG yi-fang, GU chun-mei. Department of Obstetric, Women and Children’s Health Care Hospital, Yangzhou city 225000,China
【Abstract】 Objective To research the hepatitis B virus carrying rate from the breast milk of the hepatitis B virus-carried mothers and to analyze their relationship and the probability of breast-feeding. Methods Hepatitis B virus markers in breast milk of 42 HBV carrier mothers were detected by ELISA. HBV DNA in serum was tested with the real-time quantitative polymerase chain reaction.42 women who were not infected were contrast. Results HBsAg positive rate in study group was 33.3% in the breast milk. HBV-DNA positive rate in study group was 28.57% in maternal serum. The rates of the control group were zero. Conclusion Mothers with all the three markers of HBsAg, Anti-HbeAb and Anti-HbcAb positive have low rate of virus detection, and they are relatively safe for breast-feeding. Their breast milk has low rate of infections than the breast milk coming from the mother with the three markers of HBsAg, HbeAg and Anti-HbcAb positive. It is same to the mother with the marker of HBsAg positive.
【Key words】Hepatitis B virus; Human; Breast-feeding
乙型肝炎是一种严重危害人类健康的世界性传染病，大约30%的世界人口，约20亿人感染了乙肝[1]。其中，约有3.5亿人感染了慢性乙肝，每年约有100万慢性乙肝患者死亡[2]。我国是乙肝高发区，乙肝表面抗原携带者高达10%~15%[3]。母婴传播是乙肝病毒的主要传播途径之一。关于乙肝血清学指标阳性的母亲能否哺乳，国内外争议较大。鉴于母乳喂养的优越性，且近年来世界卫生组织将保护、促进和支持母乳喂养作为世界儿童保健工作的重要内容，因而乙肝血清学指标阳性的母亲是否适于哺乳的问题值得进一步研究。本研究通过检测乙肝血清学指标阳性的产妇乳汁中乙肝两对半及外周血HBV-DNA定量，以探讨两者之间的关系及其对于母乳喂养的影响。
1 资料与方法
1.1 一般资料 选择本院产科2010年1月至2010年12月住院分娩的乙型肝炎产妇42例。检测产妇产后3 d内的乳汁中乙肝两对半，同时检测外周血HBV-DNA定量。另取同期42例产前静脉血乙肝两对半全阴的产妇作为对照组。同样取其产后3 d的乳汁进行乙肝两对半及外周血HBV-DNA检测。
1.2 方法
1.2.1 试剂来源 乙肝两对半ELISA试剂盒由北京万泰生物制品有限公司提供 ......
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